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人外周血淋巴細胞

簡要描述:CCL-120 CCRF-SD 人外周血淋巴細胞
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  • 產品型號:CCRF-SD
  • 廠商性質:生產廠家
  • 更新時間:2024-11-12
  • 訪  問  量:2058

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CCL-120 CCRF-SD 人外周血淋巴細胞

ATCC® Number: CCL-120™ Price: $338.00

Designations: CCRF-SB [CCRF SB]

Depositors: GE Foley

Biosafety Level: 2 [Cells Contain HERPESVIRUS ]

Shipped

: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens (human


Morphology: lymphoblast

CCL-120 CCRF-SD 人外周血淋巴細胞

Source: Organ: peripheral blood

Disease: acute lymphoblastic leukemia

Cell Type: B lymphoblast;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Antigen Expression: HLA A1, A2, B12, B17, Cw2

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 10,12

D16S539: 9,13

D5S818: 11,12

D7S820: 11,12

THO1: 9,10

TPOX: 8

vWA: 18

Isoenzymes: G6PD, B

Age: 11.5 years

Gender: male

Ethnicity: Caucasian

Comments: A T cell line (HSB-2, ATCC CCL-120.1) was also obtained from the same buffy coat preparation.

Derived from a buffy coat preparation from a patient with ALL.

The cells are EBNA positive.

Propagation: ATCC complete growth medium: RPMI 1640 medium, 90%; fetal bovine serum, 10% - OR - Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 3 X 10(5) viable cells/ml.

Medium Renewal: Add fresh medium (10% to 20% by volume) every 2 to 3 days

Related Products: derived from same individual:ATCC CCL-120.1

References: 24413: . M.D. Anderson Hospital and Tumor Inst. Monograph. 21: ; 1967.

25967: Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381



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